Biomarker Technologies Publishes Best Practices for Spatial Transcriptomics Sample Prep

Biomarker Technologies (BMKGene) has published a detailed guide outlining best practices for sample preparation in spatial transcriptomics. The guidance highlights the critical role of sample preparation in ensuring high-quality and reliable experimental outcomes.

Spatial transcriptomics allows researchers to study gene expression while preserving the spatial organization of cells within tissues. Unlike traditional RNA sequencing, it offers deeper insights into tissue architecture, disease progression, and cellular interactions. However, the success of a spatial transcriptomics experiment hinges on meticulous sample preparation, as even minor handling variations can significantly impact RNA quality, tissue morphology, and downstream data.

BMKGene's guide covers key considerations such as tissue dimensions, preparing backup samples, maintaining a cold and RNase-free environment, and rapid tissue freezing and OCT embedding. Emphasis is placed on the importance of swift handling to prevent RNA degradation and preserve cellular structures, noting that slow freezing can lead to artifacts that compromise analysis.

The publication aims to help researchers avoid common pitfalls and generate more robust and reproducible spatial transcriptomics data. Careful planning and execution during sample preparation are presented as essential for enhancing the quality and reliability of spatial transcriptomics studies.